Carbon Nanotube-Based Biosensors For DNA Sequencing

Authors

  • Omar Sebastián Muñoz Merino Escuela Superior Politécnica de Chimborazo orcid.org /0009-0002-0933-4966 Riobamba-Ecuador
  • Milton Jhoel Villacrés Vega Escuela Superior Politécnica de Chimborazo orcid.org /0009-0000-7677-4764 Riobamba-Ecuador
  • José Luis Cortés Llanganate Escuela Superior Politécnica de Chimborazo https:// orcid.org /0000-0002-3228-2669 Riobamba-Ecuador
  • Bryan Nicolas Merino Barreno Escuela Superior Politécnica de Chimborazo https:// orcid.org /0009-0004-8049-5775\ Riobamba-Ecuador

DOI:

https://doi.org/10.29284/12d4ta21

Keywords:

Sequencing, DNA, Biosensor, Nanotube, Hybridization , Oligonucleotides.

Abstract

DNA sequencing has enormous potential for biology and medicine, especially for a personalized diagnosis and treatment. Today, however, sequencing technology remains slow and expensive, elements prohibitive for most clinical applications. The development of biosensors based on carbon nanotubes will allow us to detect genetic sequences thanks to the electrical signal that is generated when two complementary DNA strands are joined (hybridization). Next, the most relevant aspects of the operation of the same will be detailed and it is necessary to emphasize that our work is purely theoretical in order that in the future it will serve for other researchers the experimentation of the same one.

References

1. Ashraf, A. (2016). Wettability of graphitic materials and their application in separation [PhD Thesis, University of Illinois at Urbana-Champaign]. https://www.ideals.illinois.edu/items/93223

2. Belytschko, T., Xiao, S. P., Schatz, G. C., & Ruoff, R. S. (2002). Atomistic simulations of nanotube fracture. Physical Review B, 65(23), 235430. https://doi.org/10.1103/PhysRevB.65.235430

3. Biémont, C., Vieira, C., Borie, N., & Lepetit, D. (1999). Transposable elements and genome evolution: The case of Drosophila simulans. Genetica, 107(1–3), 113–120. https://doi.org/10.1023/A:1003937603230

4. Boulle, O., Vogel, J., Yang, H., Pizzini, S., de Souza Chaves, D., Locatelli, A., Menteş, T. O., Sala, A., Buda-Prejbeanu, L. D., & Klein, O. (2016). Room-temperature chiral magnetic skyrmions in ultrathin magnetic nanostructures. Nature nanotechnology, 11(5), 449–454.

5. Brumfiel, G. (2009). Nanotubes cut to ribbons. Nature News. https://www.natureasia.com/static/ja-jp/ndigest/pdf/v6/n6/ndigest.2009.090634.pdf

6. Camacho, M. L., Herrera, G., Ruiz, M. A., Pérez, R., García, J. E., Betancourt, J. A., & Lardizábal-Gutiérrez, D. (2023). Optimization of in situ formation of a titanium carbide nanohybrid via mechanical alloying using stearic acid and carbon nanotubes as carbon sources. Journal of Composites Science, 7(12), 502.

7. Carrillo, A. (2020). Low-temperature synthesis, enhanced reaction times and characterization of TiO2 nanoparticles by a microwave assisted sol-gel method for its application in green industry. Instituto de Ingeniería y Tecnología. https://cathi.uacj.mx/handle/20.500.11961/16037

8. Carrión, F. J., Espejo, C., Sanes, J., & Bermúdez, M. D. (2010). Single-walled carbon nanotubes modified by ionic liquid as antiwear additives of thermoplastics. Composites science and technology, 70(15), 2160–2167.

9. Chakraborty, T., Roy, P., & Nesa, N. (2023). IoMT-Based Biochip-Integrated Point-of-Care Testing for Smart Healthcare. IEEE internet of things journal, 10(24), 22740–22747.

10. Chen, T., D’Addio, S. M., Kennedy, M. T., Swietlow, A., Kevrekidis, I. G., Panagiotopoulos, A. Z., & Prud’homme, R. K. (2009). Protected Peptide Nanoparticles: Experiments and Brownian Dynamics Simulations of the Energetics of Assembly. Nano Letters, 9(6), 2218–2222. https://doi.org/10.1021/nl803205c

11. Collins, P. G., & Avouris, P. (2000). Nanotubes for electronics. Scientific american, 283(6), 62–69.

12. Conroy, P. J., Hearty, S., Leonard, P., & O’Kennedy, R. J. (2009). Antibody production, design and use for biosensor-based applications. Seminars in cell & developmental biology, 20(1), 10–26. https://www.sciencedirect.com/science/article/pii/S1084952109000147

13. Daboussi, M. J. (1997). Fungal transposable elements and genome evolution. Genetica, 100(1–3), 253–260. https://doi.org/10.1023/A:1018354200997

14. Duda, A., & Tarko, T. (2023). Possible side effects of polyphenols and their interactions with medicines. Molecules, 28(6), 2536.

15. Eder, D. (2010). Carbon Nanotube−Inorganic Hybrids. Chemical Reviews, 110(3), 1348–1385. https://doi.org/10.1021/cr800433k

16. Finnegan, D. J. (1989). Eukaryotic transposable elements and genome evolution. Trends in genetics, 5, 103–107.

17. Frías, I. A. M., Avelino, K. Y. P. S., Silva, R. R., Andrade, C. A. S., & Oliveira, M. D. L. (2015). Trends in Biosensors for HPV: Identification and Diagnosis. Journal of Sensors, 2015, 1–16. https://doi.org/10.1155/2015/913640

18. Galperin, M. Y., & Koonin, E. V. (2010). From complete genome sequence to ‘complete’understanding? Trends in biotechnology, 28(8), 398–406.

19. Genereux, J. C., & Barton, J. K. (2010). Mechanisms for DNA Charge Transport. Chemical Reviews, 110(3), 1642–1662. https://doi.org/10.1021/cr900228f

20. Herrera, A. (2021). Design and development of molecularly imprinted polymers for electronic tongues and biosensing. https://ddd.uab.cat/record/264824

21. Huhtala, M., Kuronen, A., & Kaski, K. (2002). Carbon nanotube structures: Molecular dynamics simulation at realistic limit. Computer Physics Communications, 146(1), 30–37.

22. Hwang, M. T., Landon, P. B., Lee, J., Choi, D., Mo, A. H., Glinsky, G., & Lal, R. (2016). Highly specific SNP detection using 2D graphene electronics and DNA strand displacement. Proceedings of the National Academy of Sciences, 113(26), 7088–7093. https://doi.org/10.1073/pnas.1603753113

23. James, T. D., Mulvaney, P., & Roberts, A. (2016). The Plasmonic Pixel: Large Area, Wide Gamut Color Reproduction Using Aluminum Nanostructures. Nano Letters, 16(6), 3817–3823. https://doi.org/10.1021/acs.nanolett.6b01250

24. Jasti, R., Bhattacharjee, J., Neaton, J. B., & Bertozzi, C. R. (2008). Synthesis, Characterization, and Theory of [9]-, [12]-, and [18]Cycloparaphenylene: Carbon Nanohoop Structures. Journal of the American Chemical Society, 130(52), 17646–17647. https://doi.org/10.1021/ja807126u

25. Jastrzębska, A. M., Kurtycz, P., & Olszyna, A. R. (2012). Recent advances in graphene family materials toxicity investigations. Journal of Nanoparticle Research, 14(12), 1320. https://doi.org/10.1007/s11051-012-1320-8

26. Jorda, J., Xue, B., Uversky, V. N., & Kajava, A. V. (2010). Protein tandem repeats – the more perfect, the less structured. The FEBS Journal, 277(12), 2673–2682. https://doi.org/10.1111/j.1742-4658.2010.07684.x

27. Jurka, J. (2007). Conserved eukaryotic transposable elements and the evolution of gene regulation. Cellular and Molecular Life Sciences: CMLS, 65(2), 201.

28. Kamat, P. V. (2006). Harvesting photons with carbon nanotubes. Nano Today, 1(4), 20–27.

29. Khalaj, Z., Monajjemi, M., & Diudea, M. V. (2017). Main allotropes of carbon: A brief review. Sustainable Nanosystems Development, Properties, and Applications, 185–213.

30. Khan, A. S. (2012). Nanotechnology: An introduction to applications and ethical and social issues. Nanotechnology: ethical and social implications. CRC Press, Boca Raton, FL. https://api.taylorfrancis.com/content/chapters/edit/download?identifierName=doi&identifierValue=10.1201/b12005-6&type=chapterpdf

31. Kowalczyk, S. W., Blosser, T. R., & Dekker, C. (2011). Biomimetic nanopores: Learning from and about nature. Trends in biotechnology, 29(12), 607–614.

32. Kumar, S., Cola, B. A., Jackson, R., & Graham, S. (2011a). A review of carbon nanotube ensembles as flexible electronics and advanced packaging materials. Journal of Electronic Packaging, 133(2). https://asmedigitalcollection.asme.org/electronicpackaging/article-abstract/133/2/020906/405044

33. Kumar, S., Cola, B. A., Jackson, R., & Graham, S. (2011b). A review of carbon nanotube ensembles as flexible electronics and advanced packaging materials. Journal of Electronic Packaging, 133(2). https://asmedigitalcollection.asme.org/electronicpackaging/article-abstract/133/2/020906/405044

34. Ladame, S., & Chang, J. (2019). Bioengineering innovative solutions for cancer. Academic Press. https://books.google.com/books?hl=es&lr=&id=D7rADwAAQBAJ&oi=fnd&pg=PP1&dq=16.%09MIT+Technology+Review+.+Nanotube+Biosensors+for+Detecting+Cancer-Associated+Molecules.+MIT+Technology+Review+%3B+2012.&ots=8KHRFoWE4X&sig=jw9eSVaYESht3bTaIRSSQeREwqE

35. Langer, R. (1995). Biomaterials and biomedical engineering. Chemical Engineering Science, 50(24), 4109–4121.

36. Layqah, L. A., & Eissa, S. (2019). An electrochemical immunosensor for the corona virus associated with the Middle East respiratory syndrome using an array of gold nanoparticle-modified carbon electrodes. Microchimica Acta, 186(4), 224. https://doi.org/10.1007/s00604-019-3345-5

37. Li, J., Ng, H. T., Cassell, A., Fan, W., Chen, H., Ye, Q., Koehne, J., Han, J., & Meyyappan, M. (2003). Carbon Nanotube Nanoelectrode Array for Ultrasensitive DNA Detection. Nano Letters, 3(5), 597–602. https://doi.org/10.1021/nl0340677

38. Liang, Y., Xiao, M., Wu, D., Lin, Y., Liu, L., He, J., Zhang, G., Peng, L.-M., & Zhang, Z. (2020). Wafer-Scale Uniform Carbon Nanotube Transistors for Ultrasensitive and Label-Free Detection of Disease Biomarkers. ACS Nano, 14(7), 8866–8874. https://doi.org/10.1021/acsnano.0c03523

39. Liu, Q., Ren, W., Chen, Z.-G., Yin, L., Li, F., Cong, H., & Cheng, H.-M. (2009). Semiconducting properties of cup-stacked carbon nanotubes. Carbon, 47(3), 731–736.

40. Martín, L., Resines, E., Sánchez, J., & Burzurí, E. (2024). Graphene conductance modulation through controlled molecular release in a hybrid coordination polymer/graphene field-effect transistor. Carbon, 225, 119145.

41. Mikhailovich, V., Gryadunov, D., Kolchinsky, A., Makarov, A. A., & Zasedatelev, A. (2008). DNA microarrays in the clinic: Infectious diseases. BioEssays, 30(7), 673–682. https://doi.org/10.1002/bies.20781

42. Mishra, S., Dash, D., & Das, A. P. (2022). Detection, characterization and possible biofragmentation of synthetic microfibers released from domestic laundering wastewater as an emerging source of marine pollution. Marine pollution bulletin, 185, 114254.

43. Mora Bolaños, R. J., & Chaves Villareal, C. (2021). Evaluación del Efecto del uso de Aditivos de Carbono en las Propiedades Térmicas y Mecánicas de un Material Termoplástico con Potenciales Aplicaciones en el Sector Biomédico y Aeroespacial. https://repositorio.una.ac.cr/items/4c12d1ea-697b-4cc9-a823-4cd60675b446

44. Pêgo, A. (2023). Dendrimers and Derivatives as Multifunctional Nanotherapeutics for Alzheimer’s Disease. Pharmaceutics. https://www.academia.edu/download/124011284/pdf.pdf

45. Pop, E., Mann, D., Wang, Q., Goodson, K., & Dai, H. (2006). Thermal Conductance of an Individual Single-Wall Carbon Nanotube above Room Temperature. Nano Letters, 6(1), 96–100. https://doi.org/10.1021/nl052145f

46. Ruoff, R. S., Qian, D., & Liu, W. K. (2003). Mechanical properties of carbon nanotubes: Theoretical predictions and experimental measurements. Comptes rendus. Physique, 4(9), 993–1008.

47. Shendure, J., Balasubramanian, S., Church, G. M., Gilbert, W., Rogers, J., Schloss, J. A., & Waterston, R. H. (2017). DNA sequencing at 40: Past, present and future. Nature, 550(7676), 345–353.

48. Sireesha, M., Jagadeesh Babu, V., Kranthi Kiran, A. S., & Ramakrishna, S. (2018a). A review on carbon nanotubes in biosensor devices and their applications in medicine. Nanocomposites, 4(2), 36–57. https://doi.org/10.1080/20550324.2018.1478765

49. Sireesha, M., Jagadeesh Babu, V., Kranthi Kiran, A. S., & Ramakrishna, S. (2018b). A review on carbon nanotubes in biosensor devices and their applications in medicine. Nanocomposites, 4(2), 36–57. https://doi.org/10.1080/20550324.2018.1478765

50. Spitalsky, Z., Tasis, D., Papagelis, K., & Galiotis, C. (2010). Carbon nanotube–polymer composites: Chemistry, processing, mechanical and electrical properties. Progress in polymer science, 35(3), 357–401.

51. Star, A., Tu, E., Niemann, J., Gabriel, J.-C. P., Joiner, C. S., & Valcke, C. (2006). Label-free detection of DNA hybridization using carbon nanotube network field-effect transistors. Proceedings of the National Academy of Sciences, 103(4), 921–926. https://doi.org/10.1073/pnas.0504146103

52. Subramanian, B. (2013). Enhancement of biocompatibility of metal implants by nanoscale TiN/NbN multilayer coatings. Journal of Nanoscience and Nanotechnology, 13(7), 4565–4572.

53. Terrones, H. (2013). Fullerenes and Beyond: Complexity, Morphology, and Functionality in Closed Carbon Nanostructures. En R. Vajtai (Ed.), Springer Handbook of Nanomaterials (pp. 83–104). Springer Berlin Heidelberg. https://doi.org/10.1007/978-3-642-20595-8_3

54. Wang, J., Li, L., Liu, H., & Hou, Q. (2024). Graphene/Carbon Nanotube Conductive Ink-Based Biomimetic Structure for Self-Powered Flexible Medical Monitoring Devices. ACS Applied Nano Materials, 7(2), 1863–1875. https://doi.org/10.1021/acsanm.3c05095

55. Yang, F., Wang, X., Zhang, D., Yang, J., Luo, D., Xu, Z., Wei, J., Wang, J.-Q., Xu, Z., & Peng, F. (2014). Chirality-specific growth of single-walled carbon nanotubes on solid alloy catalysts. Nature, 510(7506), 522–524.

56. Zhang, D. Y., Chen, S. X., & Yin, P. (2012). Optimizing the specificity of nucleic acid hybridization. Nature chemistry, 4(3), 208–214.

57. Zoli, M. (2003). Polaronic features in the Su–Schrieffer–Heeger model. Physica B: Condensed Matter, 329, 1554–1555.

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Published

2025-12-10

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How to Cite

[1]
O. S. M. Merino, M. J. V. Vega, J. L. C. Llanganate, and B. N. M. Barreno, “Carbon Nanotube-Based Biosensors For DNA Sequencing ”, IJASIS, pp. 1277–1292, Dec. 2025, doi: 10.29284/12d4ta21.

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